Biological Activities of Some Fungal Isolates From Different Marine Environments
$ 70
Description
The study concerned with 26 fungal strains collected from different places of Meditreanean sea in ( Marsa matrouh and Damietta) and Red sea in (Sharm el-sheikh, El-ain el-sokhna and El- Quseir). The antimicrobial activity against pathogenic microbes was examined. Active marine fungi were from El- Quseir and Damietta which coded 13 and 26, respectively. The cytotoxic activity of natural products of fungi 13 and 26 that extracted by ethyl acetate and petroleum ether against human hepatocellular cancer cell line (HepG2 cells), human colon carcinoma cell line (HCT-116 cells) and human breast cancer cell line (MCF-7 cells) and our findings revealed that ethyl acetate extract had the highest activity against cancer cell line.The antioxidant activity of natural products of fungi 13 and 26 were also determined using the DPPH free radical scavenging assay in triplicate.Aspergillus fumigatus and its purified active compound exerted significant antibacterial activity reaching highest potency with MIC 19.5 and 39.06µg/mL, respectively.The effect of purified antimicrobial compound from extracellular metabolites of Aspergillus fumigatus on bacterial cell structure was tested using transmission electron microscopy on Gram-negative tested bacteria Enterobacter cloacae, Gram-positive tested bacteria Staphylococcus aureus.An obvious decrease of the cell population at G1 phase and the G2/M phase of HepG2 cancer cells were detected by the purified compound when compared with untreated HepG2 control cells. Marked morphological changes were observed in HepG2 cancer cells treated with the active pure compound and the ability of tumor cells became worse.The cytotoxic effect of the purified compound was evaluated against normal cells on African Green Monkey kidney (VERO) cell line using MTT assay and non-significant cytotoxic effects were observed for the pure compound when tested at high and lower concentrations. Chromatographic separations of the active ethyl acetate extract were performed using column chromatography, TLC, Mass spectrum, IR, NMR and GCMS.